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Pro123-2

CHARACTERIZATION AND IDENTIFICATION OF ENVIRONMENTAL FUNGAL AND BACTERIAL FLORA ON THE SURFACE OF IN SITU EARTHEN MATERIALS



Author(s): A. Simons, A. Bertron, C. Roux, J.-E. Aubert and C. Roques
Paper category: Proceedings
Book title: Proceedings of the Final Conference of RILEM 253-MCI Microorganisms-Cementitious Materials Interactions Volume II
Editor(s): Alexandra Bertron and Henk Jonkers
ISBN: 978-2-35158-207-7 (Set)
ISBN: 978-2-35158-210-7 (Volume 2)
e-ISBN: 978-2-35158-206-0
Publisher: RILEM Publications SARL
Publication year: 2018
Pages: 325-334
Total Pages: 308
Language : English


Abstract: Nowadays, the impact of building materials on the health of inhabitants is a priority issue. The microbial proliferation on the surface of materials can lead to a degradation of the indoor air quality and the development of pathologies for the occupants. Raw earth materials currently experience a renewed interest due to ecological and economic reasons. A major question about raw earth materials concerns their microbial status: do such natural material inhabits microbes that could provoke inhabitants disease? Very few global analyses of microbial communities present on these supports were performed. The characterization of raw earth material microbiome is also important to better evaluate and understand the susceptibility of earthen materials to microbial development. In this manuscript, we present a study of the distribution of in situ bacterial and fungal communities on different raw earth materials used in construction. Samplings have been carried out on different buildings in France and microbial communities were characterized by DNA high-throughput sequencing. Major fungal and bacterial genera identified were mainly associated to conventional outdoor and indoor environmental communities. Interestingly, the main features influencing microbial communities are building history and room use rather than material composition. These results consist in a first deep analysis of microbial communities present on raw earthen materials for which we developed different protocols and may be considered as a cornerstone to investigate microbial communities on such material according to environmental conditions and its potential health impact.Nowadays, the impact of building materials on the health of inhabitants is a priority issue. The microbial proliferation on the surface of materials can lead to a degradation of the indoor air quality and the development of pathologies for the occupants. Raw earth materials currently experience a renewed interest due to ecological and economic reasons. A major question about raw earth materials concerns their microbial status: do such natural material inhabits microbes that could provoke inhabitants disease? Very few global analyses of microbial communities present on these supports were performed. The characterization of raw earth material microbiome is also important to better evaluate and understand the susceptibility of earthen materials to microbial development. In this manuscript, we present a study of the distribution of in situ bacterial and fungal communities on different raw earth materials used in construction. Samplings have been carried out on different buildings in France and microbial communities were characterized by DNA high-throughput sequencing. Major fungal and bacterial genera identified were mainly associated to conventional outdoor and indoor environmental communities. Interestingly, the main features influencing microbial communities are building history and room use rather than material composition. These results consist in a first deep analysis of microbial communities present on raw earthen materials for which we developed different protocols and may be considered as a cornerstone to investigate microbial communities on such material according to environmental conditions and its potential health impact.


Online publication : 2018
Publication type : full_text
Public price (Euros) : 0.00


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