Author(s): Olja Lj. Šovljanski, Ana M. Vidaković, Snežana B. Vučetić, Ivan S. Ristić, Bojan B. Miljević, Jonjaua G. Ranogajec and Siniša L. Markov
Paper category: Proceedings
Book title: Proceedings of the Final Conference of RILEM 253-MCI Microorganisms-Cementitious Materials Interactions Volume II
Editor(s): Alexandra Bertron and Henk Jonkers
ISBN: 978-2-35158-207-7 (Set)
ISBN: 978-2-35158-210-7 (Volume 2)
e-ISBN: 978-2-35158-206-0
Publisher: RILEM Publications SARL
Publication year: 2018
Pages: 591-592
Total Pages: 308
Language : English

Abstract: In recent years, extensive studies have been conducted on the application of carbonate precipitating bacteria for approaching a self-healing effect in cementitious materials. The aim of this study was to determine the number of survived spores after the encapsulation into hydrogel (ACM-co-AA (ratio 1:1), prepared by the method of microwaves, as well as to prove their placement within the pores of hydrogel. The xerogel was hydrated with suspension of spores of Sporosarcina pasteruii DSM 33 and rehydrated hydrogel was subjected to drying prior to formation of external hydrophobic capsule of hydrogel encapsulated spores by double- sided coating with an elastomer. Viability of the encapsulated spores was determined after the incubation at 30 °C for 48h on TSA with the addition of 20% urea. In order to prove the entrapment of bacterial spores into hydrogel the SEM microscopy was employed. According to the obtained results, the spores were successfully entrapped in hydrogel pores without the reduction of spore number after the encapsulation.

Online publication : 2018
Publication type : full_text
Public price (Euros) : 0.00

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