Proceedings pro123-2 : Final Conference of RILEM TC 253-MCI: Microorganisms-Cementitious Materials Interactions Volume 2

Title: Final Conference of RILEM TC 253-MCI: Microorganisms-Cementitious Materials Interactions Volume 2 Edited by: Alexandra Bertron and Henk Jonkers e-ISBN: 978-2-35158-206-0 ISBN: 978-2-35158-207-7 (Set) ISBN: 978-2-35158-210-7 (Volume 2) Pages: 308 Publication date: 2018

Infrastructures and buildings are exposed to microorganisms in a variety of contexts. Structures exposed to aggressive aqueous media containing microorganisms (waste water, soft water, fresh water, ground water, sea water, agricultural or agro-industrial environments, etc.), often concrete structures, can suffer deterioration that can be detrimental (loss of alkalinity, erosion, spalling of the concrete skin, corrosion of rebars, loss of water- or airtightness...). The deleterious effect of microorganisms, mainly bacteria and fungi, on the cementitious matrix has been found to be linked, on the one hand, with the production of aggressive metabolites (e.g. acids, CO2, and/or sulphur compounds) but also, on the other hand, with some specific, physical and chemical effects of the microorganisms themselves through the formation of biofilm on the surface. Moreover, the intrinsic properties of the cementitious matrix (physical properties, mineralogical and/or chemical composition) can also influence the biofilm characteristics.
These deteriorations lead to a significant increase in the cost of repairing structures and to loss of production income, but may also lead to pollution issues resulting from waste water leakage to the environment for example.
Also, building facades, and notably concrete external walls, can be affected by biological stains, which alter aesthetical quality of the construction, sometimes very quickly, and lead to significant cleaning costs.
In indoor environments, proliferation of bacteria and fungi on building materials is responsible for health problems through the production of microbial volatile organic compounds, allergens and toxins. The bio-receptivity of construction materials conditions the proliferation of microorganisms on their surfaces. This is a significant issue as the economic and societal consequences of bacterial proliferation inside buildings are very important.
However, in some cases, microorganisms can have beneficial effects on cementitious materials when they are used for example as a way to protect and/or repair concrete in applications such as bacteria-based engineering protective systems. New formulations of cementitious materials, incorporating selected bacteria and suitable chemical precursors, are developed with the aim to fill micro-cracks in concrete and thus improve the durability properties. Moreover, concrete with enhanced bio-receptivity are being developed, e.g. with the aim of using the buildings’ envelope to provide a higher surface of green areas (living wall systems), or to reduce the impact of marine works (artificial reefs).
More and more research efforts are devoted to these topics related to cementitious materials- microorganisms interactions within local, trans-regional on international initiatives by multidisciplinary research teams involving scientists in material science, civil engineering, microbiology, microbial ecology, and bioprocess, etc. Significant progress is made, but important scientific and technical obstacles are remaining.
RILEM 253-MCI Technical Committee (2014-2019) aimed to implement concerted approaches and comparison of research outcomes to move toward a better understanding of the phenomena and furthermore, to standardization (of test methods for example) and/or certification.
The TC was organized into four Working Groups, each group tackling a major aspect of these phenomena:
Biodeterioration / microorganisms-cement-based materials interactions in different contexts: sewers, water treatment, agricultural environments, tunnels, marine environment, nuclear waste storage, etc. and the impact on the materials’ properties (biodeterioration) and/or the structures’ performances (durability, safety, etc.)
Microbial stains on building materials and protection of materials; building materials with improved bio-receptivity (vegetal walls, reefs, etc.)
Proliferation of microorganisms (bacteria, fungi) on building materials in indoor conditions and health issues.
Bacteria-based engineering protective systems for cementitious materials (biodeposition, bacteria-based self-healing systems, etc.).
RILEM TC 253-MCI met as a Committee for the first time in May 2014, and since then the committee has held a further 10 plenary meetings, and organized an International Workshop in Delft in June 2016. Besides, numerous informal communications have been held over the years between the members of the working groups.
The TC is involved in the production of some deliverables. These will comprise a comprehensive State of the Art Report (STAR) in published book form, expected to be published next year. This Report will essentially be a compilation and synthesising of reports of the working groups. This conference is also a TC deliverable. Further, it is hoped to produce a shorter report providing a summary and practical applications, for publication in the RILEM Journals.
This conference represents the final major event of the TC. It seeks to bring together a wide range of experts – researchers, practitioners, regulators, and infrastructure managers – to share experiences and new scientific information on these problems. We are pleased that the conference has attracted 55 papers and 15 posters, which will be presented over two days in Toulouse in June 2018. These communications were organized in 4 sessions, matching the different tasks of the 253-MCI working groups: (1) Biodeterioration and microorganisms- cementitious materials interactions in various contexts, (2) Microbial stains on building materials and protection of external walls; building materials with improved bio-receptivity, (3) Proliferation of microorganisms on indoor building materials and (4) Bacteria-based engineering protective systems for cementitious materials. A Young Researcher Competition is also organized, with the aim to identify and encourage talented young scientists on these emerging topics. All the papers have been rigorously peer-reviewed by experts, primarily members of the technical committee who have specific expertise in the areas considered. Thus, we believe the conference volumes will be a valuable addition to the literature on these subjects.
We wish to acknowledge the Convenors of the Working Groups for the work they have done to facilitate the broader work of the TC, and of course the contributions of the members of the working groups.

Contents

Session 2: Microbial stains on building materials and protection of materials; building materials with improved bio-receptivity

CONCRETE WITH IMPROVED BIO-RECEPTIVITY Author(s): V. Morin, F. De Larrard, I. Dubois-Brugger, M. Horgnies, S. Duchand, S. Vacher, F. Martarèche, A. Musnier and M. Lapinski

Pages: 291-300

BIORECEPTIVITY OF CEMENTITIOUS MATERIAL IN SEA WATER: DEVELOPMENT OF A LABORATORY PROTOCOL TO CHARACTERIZE COLONIZATION Author(s): M.-A. Ferrero, M. Boutouil, H. Cuadrado and P. Claquin

Pages: 301-307

ECO-DESIGN OF MARINE STRUCTURES IN THE BAY OF AJACCIO, CORSICA Author(s): Marie Salgues, Jean-Claude Souche, Gwenn Le Saout, Alexandra Agostini and Sylvain Pioch

Pages: 309-317

IMPACT OF A THIN COATING LAYER OF CALCIUM ALUMINATE AND SULFOALUMINATE CEMENTS ON ALGAL BIOFOULING Author(s): A. Govin, P. Grosseau, C. Lors and D. Damidot

Pages: 319-322

Session 3: Proliferation of microorganisms (bacteria, fungi) on building materials in indoor conditions and health issues

CHARACTERIZATION AND IDENTIFICATION OF ENVIRONMENTAL FUNGAL AND BACTERIAL FLORA ON THE SURFACE OF IN SITU EARTHEN MATERIALS Author(s): A. Simons, A. Bertron, C. Roux, J.-E. Aubert and C. Roques

Pages: 325-334

MYCOTOXIN PRODUCTION, AEROSOLISATION AND RESISTANCE TO REMEDIATION PROCESS ON DIFFERENT INDOOR MATERIALS Author(s): Brankiça Aleksic, Sylviane Bailly, Marjorie Draghi, Enric Robine and Jean- Denis Bailly

Pages: 335-345

IMPACTS OF ALKALINITY AND ENVIRONMENTAL AGEING ON FUNGAL GROWTH IN HEMP CONCRETES Author(s): G. Delannoy, M. Guéguen-Minerbe, I. Nour, S. Marceau, P. Glé, E. Gourlay, D. Diafi, S. Amziane and F. Farcas

Pages: 347-353

ANTIBACTERIAL PROPERTIES OF A PHOTOCATALYTIC PAINT-LIKE COATING FOR THE PROTECTION OF BUILDING MATERIALS AGAINST MICROBIAL PROLIFERATION Author(s): Thomas Verdier, Alexandra Bertron and Christine Roques

Pages: 355-365

IMPROVING THE METHODOLOGY OF LABORATORY TESTS TO EXPLORE SENSITIVITY OF BUILDING MATERIALS TO FUNGAL GROWTH: CASE OF BIO- BASED EARTH MATERIALS Author(s): Aurélie Laborel-Préneron, Kouka Ouédraogo, Alexis Simons, Matthieu Labat, Alexandra Bertron, Camille Magniont, Christine Roques, Christophe Roux and Jean-Emmanuel Aubert

Pages: 367-376

Session 4: Bacteria-based engineering protective systems for cementitious materials

BIOTECHNOLOGY OFFERS MORE DURABLE AND SUSTAINABLE CEMENTITIOUS COMPOSITES Author(s): Yusuf Ç. Erşan

Pages: 379-386

APPLICATION AND PERFORMANCE OF BACTERIA-BASED SELF-HEALING CONCRETE Author(s): Kevin Paine, Trupti Sharma, Mohamed Alazhari, Andrew Heath and Richard Cooper

Pages: 387-394

A BACTERIA-BASED AGENT FOR REALIZING COST-EFFECTIVE SELF- HEALING CONCRETE IN LOW TEMPERATURE MARINE ENVIRONMENTS Author(s): Damian Palin, Mitchell A. A. Geleijnse, Ben A. Abbas, Virginie A. C. Wiktor and Henk M. Jonkers

Pages: 395-404

SCREENING OF PH-RESPONSIVE HYDROGELS FOR ENCAPSULATION OF BACTERIA FOR CONCRETE CRACK SELF-SEALING Author(s): J. Wang, A. Mignon, G. Trenson, S. Van Vlierberghe, N. Boon and N. De Belie

Pages: 405-412

ULTRASONIC MONITORING OF BACTERIAL HEALING OF CONCRETE Author(s): Nimrat Pal Kaur, Jay Kumar Shah, Subhra Majhi, Asha Ramachandran and Abhijit Mukherjee

Pages: 415-424

SELF-HEALING EFFICIENCY OF CEMENT-BASED MATERIALS CONTAINING EXTRUDED CEMENTITIOUS HOLLOW TUBES FILLED WITH BACTERIAL HEALING AGENT Author(s): Giovanni Anglani, Paola Antonaci, Jean-Marc Tulliani, Kim Van Tittelboom, Jianyun Wan and Nele De Belie

Pages: 425-431

MICROBIAL CALCITE INDUCED PRECIPITATION GROUND STRENGTHENING WITH MINIMUM CEMENT CONTENT Author(s): A.E. Richardson, R. Valentine, M. Abrahams and D. Nicholson

Pages: 433-443

CARBON SOURCE SELECTION FOR MICROBIAL CONVERSION TO MINERALS IN CEMENTITIOUS MATERIALS Author(s): Renee M. Mors and Henk M. Jonkers

Pages: 445-454

EVALUATIONS OF EFFECTS OF NUTRIENTS, CALCIUM PRECURSORS, AND BACTERIA ON MECHANICAL PROPERTIES OF MORTAR SPECIMENS Author(s): Chien-Chung Chen, Maeve B. Cucolotto, Marko Balac, Sung Wang, Chen-Hsuan Chiu and W.T. Evert Ting

Pages: 455-463

USING LIGHTWEIGHT AGGREGATES AS A CARRIER FOR BACTERIAL SPORES IN SELF-HEALING CONCRETE Author(s): Chen-Hsuan Chiu, Chien-Chung Chen and W.T. Evert Ting

Pages: 465-473

GRANULES WITH ACTIVATED COMPACT DENITRIFYING CORE (ACDC) FOR SELF-HEALING CONCRETE WITH CORROSION PROTECTION FUNCTIONALITY Author(s): Yusuf Ç. Erşan, Nico Boon and Nele De Belie

Pages: 475-484

EFFECT OF BACTERIA ON MECHANICAL PROPERTIES OF ENGINEERED CEMENTITIOUS COMPOSITES Author(s): Yuanzhao Ding, Dafang Li, Zhigang Zhang and Shunzhi Qian

Pages: 485-490

SCREENING OF SPORULATION MEDIA FOR CONCRETE HEALING BACTERIA Author(s): W. T. Evert Ting, Chen-Hsuan Chiu and Chien-Chung Chen

Pages: 491-500

APPLICABILITY OF BIO-BASED SELF-HEALING CONCRETE IN CENTRAL EUROPEAN CONDITIONS: A PRELIMINARY STUDY Author(s): Schreiberová Hana, Petr Bílý and Pavla Ryparová

Pages: 501-510

HEALING DEPTH AND FUNCTIONALITY REGAIN OF NON-AXENIC GRANULATED CULTURE BASED SELF-HEALING CONCRETE Author(s): Yusuf Ç. Erşan, Damian Palin, Henk Jonkers, Nico Boon and Nele De Belie

Pages: 511-520

MECHANISMS OF CEMENTATION OF AUSTRALIAN ROAD BASES USING MICP Author(s): Hannah Porter, Navdeep Dhami and Abhijit Mukherjee

Pages: 521-531

IMPACT OF CHEMICAL ADMIXTURES ON BIO-BASED SELF-HEALING MORTAR Author(s): Ali Amiri, Mahzad Azima and Zeynep Başaran Bundur

Pages: 533-542

A REVIEW ON UTILIZATION OF POZZOLANIC MATERIALS IN MICROBIAL CONCRETE Author(s): Xuejiao Zhu, Nele De Belie and Varenyam Achal

Pages: 543-549

USE OF MICROORGANISMS TO IMPROVE RHEOLOGY OF CEMENT-BASED MATERIALS Author(s): Mahzad Azima, Arda Sepetçi, Şeyma Gürel and Zeynep Basaran Bundur

Pages: 551-558

Poster session

INFLUENCE OF PHYSICO-CHEMICAL PROPERTIES OF CEMENT BINDERS ON THEIR BIODETERIORATION IN SEWER NETWORK CONDITIONS Author(s): A. Buvignier, M. Peyre Lavigne, C. Patapy, E. Paul and A. Bertron

Pages: 561-562

PERFORMANCE OF ALTERNATIVE CEMENTITIOUS BINDERS TO BIOLOGICAL ATTACK IN SEWER NETWORKS. Author(s): Amr Aboulela, Matthieu Peyre-Lavigne, Cédric Patapy, Samuel Meulneyzer, Fabrizio Moro and Alexandra Bertron

Pages: 563-564

THE SPECIFIC ROLE OF MICROBIAL BIOFILMS IN THE DETERIORATION OF CONCRETE TANKS FOR ANAEROBIC DIGESTION PLANTS Author(s): Cedric Perez, Christine Lors and Benjamin Erable

Pages: 565-566

STUDY OF BACTERIAL ANOXIC CORROSION IN CEMENTITIOUS ENVIRONMENT Author(s): S. Grousset, C. Wittebroodt, A. Dauzères and L. Urios

Pages: 567-568

MICROBIAL HYDROGENOTROPHIC DENITRIFICATION IN NUCLEAR WASTE REPOSITORY CONTEXT - EFFECT OF SOLID CEMENT PASTE PRESENCE Author(s): Pierre Albina, Nadège Durban, Alexandra Bertron, Achim Albrecht, Jean- Charles Robinet, Benjamin Erable

Pages: 569-570

EVALUATION OF THE BIOLOGICAL SULPHATE REDUCTION COUPLED WITH PROPIONATE OXYDATION IN CEMENTITIOUS ENVIRONMENT Author(s): Nadège Durban, Pierre Albina, Alexandra Bertron, Achim Albrecht, Jean- Charles Robinet, Benjamin Erable

Pages: 571-573

COMPARISON OF BIOCIDES, ALLELOPATHIC SUBSTANCES AND UV-C AS TREATMENTS FOR BIOFILM PROLIFERATION ON HERITAGE MONUMENTS Author(s): Pfendler S., Borderie F., Alaoui-Sehmer L., Bousta F., Aleya L. and Alaoui- Sossé B.

Pages: 575-576

UP-SCALING AND INDUSTRIAL VALIDATION OF ENHANCED BIORECEPTIVITY MORTAS FOR LIVING FAÇADES DEVELOPMENT Author(s): Ignacio Segura, Sandra Manso, Gerard Faneca, Antonello Pitari, Pere Borralleras and Antonio Aguado

Pages: 577

INFRARED THERMOGRAPHY FOR IDENTIFICATION HYDRO AND THERMO ISOLATING DEFECTS OF BUILDINGS IN RELATION TO MOLDS Author(s): Pavel Buchta and Ilona Kukletová

Pages: 579

MONOGLYCERIDES TO PROTECT BIO-BASED INSULATION MATERIALS FROM MICROBIAL GROWTH Author(s): Thomas Verdier, Alexandra Bertron, Camille Magniont, Mathilde Montibus and Philippe Evon

Pages: 581

BIOMIMETIC MULTI-SCALE DAMAGE IMMUNITY FOR CONCRETE: THE CONTRIBUTION OF BACTERIA-BASED SELF-HEALING Author(s): Kevin Paine, Andrew Heath, Richard Cooper and Susanne Gebhard

Pages: 583

DEVELOPMENT AND EVALUATION OF A MICROORGANISM-BASED ADMIXTURE (MBA) IN MATERIALS CEMENTITIOUS Author(s): Yaset Rodríguez Rodríguez, José Fernando Martirena Hernández, Alina Hereira Díaz, Yelenys Alvarado-Capó, Yosvany Díaz-Cárdenas and Raúl González López

Pages: 585

SELECTION AND CHARACTERIZATION OF NEW BACTERIAL ISOLATES FOR CARBONATE PRECIPITATION IN SELF-HEALING CONCRETE Author(s): Bianca J. Reeksting, Kevin Paine and Susanne Gebhard

Pages: 587-588

CELL-FREE BIO-CEMENTATION FOR SOILS Author(s): Dimitrios Terzis and Laloui Lyesse

Pages: 589-590

ACRYLIC-BASED HYDROGEL ENCAPSULATED SPORES OF SPOROSARCINA PASTEURII FOR SELF-HEALING CEMENTITIOUS MATERIALS Author(s): Olja Lj. Šovljanski, Ana M. Vidaković, Snežana B. Vučetić, Ivan S. Ristić, Bojan B. Miljević, Jonjaua G. Ranogajec and Siniša L. Markov

Pages: 591-592